The membrane composite with silver nanoparticles for fibroblastic cell growth sustaining

The aim of the study was to develop the system of immobilized cells by using membranes with incorporated bactericidal component, which could be employed in biomedical applications. In this paper, we present assessment of the usability of a system of fibroblastic cells immobilized within composite membrane with commercial silver nanoparticles (AgNPs). We have assessed the basic properties of designed membrane such as water contact angle and permeability. It was observed that there was no significant difference between AgNPs-modified collagen membranes and the control-collagen membrane (p > 0.05). The mean contact angle value for all assessed composite membranes was equal to 84.13 +/- 2.20. Moreover, we have evaluated the membrane influence on the selected fibroblastic cell line. The designed AgNPs composite membrane exhibited influence on cell mitochondrial activity, however, only the composite containing 25 ppm caused above 50% functionality decline. Designed material exhibiting transport properties allowing for permeation of nutrients and metabolites. The applied membrane composites with AgNPs at concentration 6.25 up to 12.5 ppm, may be recommended for biomedical applications, especially to sustain cell growth in the skin rebuilding bandages.