Journal
CELL CYCLE
Volume 17, Issue 2, Pages 250-262Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2017.1417708
Keywords
Alcoholic liver disease; ALD; microRNA; miR-200a; ZEB2; Apoptosis
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Funding
- National Natural Science Foundation of China [81473268]
- Anhui University of Science and Technology [1704a0802161]
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ABSTRATAlcoholic liver disease (ALD) and its complication continued to be a major health problem throughout the world. Increasing evidence suggests that microRNA (miRNA) that regulate apoptosis, inflammation and lipid metabolism are affected by alcohol in ALD. MiR-200a has emerged as a major regulator in several liver diseases, but its role in ALD has not been elucidated. The aim of this study is to figure out the biological function of miR-200a in ALD and to explore its underlying mechanism. The expression pattern of miR-200a were analyzed in vitro and in vivo, we showed that miR-200a was up-regulated in ALD in AML-12 and primary hepatocyte. We then examined it's effect on cell apoptosis and identified zinc finger E-box binding homeobox 2 (ZEB2; also known as SIP1) as a direct target gene of miR-200a. Furthermore, reintroduction of ZEB2 could reverse the pro-apoptosis of miR-200a on AML-12. Taken together, our study demonstrated that miR-200a regulates the apoptosis of hepatocyte in ALD by directly target ZEB2, both of which could serve as new therapeutic targets for ALD.
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