Journal
BIOIMPACTS
Volume 8, Issue 1, Pages 53-58Publisher
TABRIZ UNIV MEDICAL SCIENCES & HEALTH SERVICES
DOI: 10.15171/bi.2018.07
Keywords
Monoacetyl morphine; Metabolites; Chemiluminescence immunoassay; Antibody; Horseradish peroxidase
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Funding
- Central Forensic Science Laboratory (CFSL), Chandigarh, India
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Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples. Methods: To develop robust immunoassay, monoacyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples. Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/mL in spiked urine samples and the limit of detection were 80, 95, 90, 75 pg/mL. Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs
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