Journal
ENVIRONMENTAL ENTOMOLOGY
Volume 45, Issue 1, Pages 66-73Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ee/nvv153
Keywords
Anoplophora glabripennis; delta C-13 fingerprint; gut microbiota; xylophagy; EAA
Categories
Funding
- Alphawood Foundation, Chicago, IL
- USDA National Institute of Food and Agriculture (NIFA) [2015-67013-23287]
- DFG-funded Cluster of Excellence The Future Ocean
Ask authors/readers for more resources
Insects are unable to synthesize essential amino acids (EAAs) de novo, thus rely on dietary or symbiotic sources for them. Wood is a poor resource of nitrogen in general, and EAAs in particular. In this study, we investigated whether gut microbiota of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), a cerambycid that feeds in the heartwood of healthy host trees, serve as sources of EAAs to their host under different dietary conditions. delta C-13-stable isotope analyses revealed significant delta C-13-enrichment (3.4 +/- 0.1%; mean +/- SEM) across five EAAs in wood-fed larvae relative to their woody diet. delta C-13 values for the consumers greater than 1% indicate significant contributions from non-dietary EAA sources (symbionts in this case). In contrast, delta C-13-enrichment of artificial diet-fed larvae (controls) relative to their food source was markedly less (1.760.1%) than was observed in wood-fed larvae, yet still exceeded the threshold of 1%. A predictive model based on delta C-13(EAA) signatures of five EAAs from representative bacterial, fungal, and plant samples identified symbiotic bacteria and fungi as the likely supplementary sources of EAA in wood-fed larvae. Using the same model, but with an artificial diet as the dietary source, we identified minor supplementary bacterial sources of EAA in artificial diet-fed larvae. This study highlights how microbes associated with A. glabripennis can serve as a source of EAAs when fed on nutrient-limited diets, potentially circumventing the dietary limitations of feeding on woody substrates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available