Journal
JOURNAL OF EXTRACELLULAR VESICLES
Volume 7, Issue 1, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/20013078.2018.1470442
Keywords
Prostate; urine; exosomes; metabolomics; metabolism; biomarkers
Categories
Funding
- ISCIII [PI12/01604]
- Spanish Ministry of Economy and Competitiveness MINECO [SAF2015-66312]
- GAP1 Movember Foundation
- department of education of the Basque Government [IKERTALDE] [IT1106-16]
- BBVA foundation
- MINECO [SAF2016-79381-R]
- European Research Council [336343, PoC 754627]
- FEDER funds
- Fundacion Vasca de Innovacion e Investigacion Sanitarias, BIOEF [BIO15/CA/052]
- AECC J.P. Bizkaia
- Basque Department of Health [2016111109]
- MINECO for REDIEX (Spanish Excellence Network in Exosomes)
- Severo Ochoa Excellence Accreditation [SEV-2016-0644]
Ask authors/readers for more resources
Urine contains extracellular vesicles (EVs) that concentrate molecules and protect them from degradation. Thus, isolation and characterisation of urinary EVs could increase the efficiency of biomarker discovery. We have previously identified proteins and RNAs with differential abundance in urinary EVs from prostate cancer (PCa) patients compared to benign prostate hyperplasia (BPH). Here, we focused on the analysis of the metabolites contained in urinary EVs collected from patients with PCa and BPH. Targeted metabolomics analysis of EVs was performed by ultrahigh- performance liquid chromatography-mass spectrometry. The correlation between metabolites and clinical parameters was studied, and metabolites with differential abundance in PCa urinary EVs were detected and mapped into cellular pathways. We detected 248 metabolites belonging to different chemical families including amino acids and various lipid species. Among these metabolites, 76 exhibited significant differential abundance between PCa and BPH. Interestingly, urine EVs recapitulated many of the metabolic alterations reported in PCa, including phosphathidylcholines, acyl carnitines, citrate and kynurenine. Importantly, we found elevated levels of the steroid hormone, 3beta-hydroxyandros-5-en-17-one-3-sulphate (dehydroepiandrosterone sulphate) in PCa urinary EVs, in line with the potential elevation of androgen synthesis in this type of cancer. This work supports urinary EVs as a non-invasive source to infer metabolic changes in PCa.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available