Journal
PROCESS BIOCHEMISTRY
Volume 52, Issue -, Pages 238-242Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2016.10.004
Keywords
Cupriavidus basilensis B-8; Kraft lignin; Lignin depolymerization; PHA production
Categories
Funding
- National Natural Science Foundation of China [31400115]
Ask authors/readers for more resources
This work presents here a new fundamental strategy for bio-converting Kraft lignin (KL) into useful products. Cupriavidus basilensis B-8 (here after B-8) was able to use KL as the sole carbon source. Fully 41.5% of lignin, 37.7% of total carbon (TC) and 43.0% of color were removed after 7 days of incubation. At the same time, lignin was depolymerized into small fragments, which was confirmed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). Bacterial biomass accumulated to 735.6 mg/L at the initial KL concentration of 5 g L-1, and the corresponding volumetric productivity of polyhydroxyalkanoate (PHA) was 128 mg/L. PHA productivity was significantly improved through fed batch fermentation and reached to 319.4 mg/L. GC-MS analysis showed that PHA polymer was composed of three basic monomers: 98.3 mol% of (S)-3-hydroxy-butanoic acid (S3HB), 1.3 mol% of (R) - ID3-hydroxybutyric acid (R3HB) and 0.4 mol% of 3-hydroxy-butanoic acid (3HB). (C) 2016 Published by Elsevier Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available