Journal
PROCESS BIOCHEMISTRY
Volume 58, Issue -, Pages 224-229Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.04.026
Keywords
Amino acids; Bacillomycin D production; Fed-batch fermentation; Gene expression
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Funding
- National Natural Science Foundation of China [31571887]
- Jiangsu Collaborative Innovation Center of Meat Production and Processing (2011 plan)
- Key Supportive Program of Excellent Youth Talent of Colleges and Universities of Anhui Province [gxyqZD2016358]
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The present study investigated the stimulation effect of L-glutamine (L-gln) on bacillomycin D biosynthesis of Bacillus subtilis fmbJ and the transcription of the synthetase genes and the signal proteins that related to the biosynthesis of bacillomycin D. It was found that L-gln significantly increased the production of bacillomycin D, in comparison with other amino acids. L-gln significantly enhanced the expression level of bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and up-regulated DegU, o(H) and SpoOA, but down-regulated AbrB, therefore the yield of bacillomycin D significantly increased. Furthermore, when conjointly feeding 5 g/L of L-gin and 30 g/L of inulin, the production of bacillomycin D was up to 1.93 g/L.
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