Journal
PROCESS BIOCHEMISTRY
Volume 54, Issue -, Pages 67-72Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.01.011
Keywords
Phospholipase; Enzymatic degumming; Site-directed mutation; Phosphatidylethanolamine removal
Categories
Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT20140951]
- Consejo Nacional de Investigaciones Cientificas y Tecnologicas, Argentina [PIP0638]
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Enzymatic phospholipid removal (degumming) is a fast-growing and environmentally friendly process for vegetable oil refining. Type C phospholipases (PLC) are the preferred enzymes since they provide an extra yield in the oil recovery. Bacillus cereus PLC can hydrolyze phosphatidylcholine (PC) but has a limited efficiency at removing phosphatidylethaholamine (PE), which together represent similar to 70% of the phospholipids present in crude soybean oil. In the present work, we show that the B. cereus PLC mutant F66Y can remove up to 90% of PE while retaining its efficiency at hydrolyzing PC. Oil treatment with the engineered enzyme provides an extra yield of 1.84% making the B. cereus PLC F66Y mutant an attractive candidate for its industrial use. (C) 2017 Elsevier Ltd. All rights reserved.
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