Journal
PROCESS BIOCHEMISTRY
Volume 62, Issue -, Pages 37-47Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.07.019
Keywords
Biodiesel; Lipases; Rhizopus microsporus; Solid-state fermentation; Packed-bed bioreactor; Scale-up
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Funding
- Universal grant from CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), a Brazilian government agency for the advancement of science and technology
- CNPq
- Fundacao Araucaria
- science and technology in the State of Parana
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior), a Brazilian government agency for the development of personnel in higher education
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A low-cost lipase preparation is required for enzymatic biodiesel synthesis. One possibility is to produce the lipase in solid-state fermentation (SSF) and then add the fermented solids (FS) directly to the reaction medium for biodiesel synthesis. In the current work, we scaled up the production of FS containing the lipases of Rhizopus microsporus. Initial experiments in flasks led to a low-cost medium containing wheat bran and sugarcane bagasse (50:50 w/w, dry basis), supplemented only with urea. We used this medium to scale-up production of FS, from 10 g in a laboratory column bioreactor to 15 kg in a pilot packed-bed bioreactor. This is the largest scale yet reported for lipase production in SSF. During scale-up, the hydrolytic activity of the FS decreased 57%: from 265 U g(-1) at 18 h in the laboratory bioreactor to 113 U g(-1) at 20 h in the pilot bioreactor. However, the esterification activity decreased by only 14%: from 12.1 U g(-1) to 10.4 U g(-1). When the FS produced in the laboratory and pilot bioreactors were dried and added directly to a solvent-free reaction medium to catalyze the esterification of oleic acid with ethanol, both gave the same ester content, 69% in 48 h.
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