Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 114, Issue 34, Pages E7045-E7053Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1708380114
Keywords
Janus particles; onion-like vesicles; self-sorting; fusion mechanism
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Funding
- National Science Foundation (NSF) [DMR-1066116, DMR-1120901]
- P. Roy Vagelos Chair at the University of Pennsylvania
- Humboldt Foundation
- NSF [DMR-1120901]
- NIH [R01 GM097552]
- University of Pennsylvania Postdoctoral Opportunities in Research and Teaching (PENN-PORT) fellowship - National Institute of General Medical Sciences [5 K12 GM081259-09]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1609784, 1066116] Funding Source: National Science Foundation
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A three-component system of Janus dendrimers (JDs) including hydrogenated, fluorinated, and hybrid hydrogenated-fluorinated JDs are reported to coassemble by film hydration at specific ratios into an unprecedented class of supramolecular Janus particles (JPs) denoted Janus dendrimersomes (JDSs). They consist of a dumbbell-shaped structure composed of an onion-like hydrogenated vesicle and an onion-like fluorinated vesicle tethered together. The synthesis of dye-tagged analogs of each JD component enabled characterization of JDS architectures with confocal fluorescence microscopy. Additionally, a simple injection method was used to prepare sub-micron JDSs, which were imaged with cryogenic transmission electron microscopy (cryo-TEM). As reported previously, different ratios of the same three-component system yielded a variety of structures including homogenous onion-like vesicles, core-shell structures, and completely self-sorted hydrogenated and fluorinated vesicles. Taken together with the JDSs reported herein, a self-sorting pathway is revealed as a function of the relative concentration of the hybrid JD, which may serve to stabilize the interface between hydrogenated and fluorinated bilayers. The fission-like pathway suggests the possibility of fusion and fission processes in biological systems that do not require the assistance of proteins but instead may result from alterations in the ratios of membrane composition.
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