Journal
GENOME BIOLOGY
Volume 19, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13059-018-1443-z
Keywords
Cas9-adenosine deaminase; sgRNA forms; Rice; Wheat; Herbicide resistance
Funding
- National Natural Science Foundation of China [31788103, 31420103912]
- National Key Research and Development Program of China [2016YFD0101804]
- Chinese Academy of Sciences [QYZDY-SSW-SMC030, GJHZ1602]
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Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A center dot T to G center dot C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.
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