Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 114, Issue 33, Pages E6992-E7001Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1708240114
Keywords
cyclin-dependent kinase 5; neurodevelopmental disorders; PSD-95; synaptic plasticity; neuronal activity
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Funding
- Research Grants Council of Hong Kong Special Administrative Region [HKUST 660213, HKUST 661013, HKUST 16124616, HKUST12/CRF/13G]
- National Key Basic Research Program of China [2013CB530900]
- Hong Kong Research Grants Council [T13-607/12R]
- Area of Excellence of the University Grants Committee [AoE/M-604/16]
- Shenzhen Peacock Plan
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The experience-dependent modulation of brain circuitry depends on dynamic changes in synaptic connections that are guided by neuronal activity. In particular, postsynaptic maturation requires changes in dendritic spine morphology, the targeting of postsynaptic proteins, and the insertion of synaptic neurotransmitter receptors. Thus, it is critical to understand how neuronal activity controls postsynaptic maturation. Here we report that the scaffold protein liprin alpha 1 and its phosphorylation by cyclin-dependent kinase 5 (Cdk5) are critical for the maturation of excitatory synapses through regulation of the synaptic localization of the major postsynaptic organizer postsynaptic density (PSD)-95. Whereas Cdk5 phosphorylates liprin alpha 1 at Thr701, this phosphorylation decreases in neurons in response to neuronal activity. Blockade of liprin alpha 1 phosphorylation enhances the structural and functional maturation of excitatory synapses. Nanoscale superresolution imaging reveals that inhibition of liprin alpha 1 phosphorylation increases the colocalization of liprin alpha 1 with PSD-95. Furthermore, disruption of liprin alpha 1 phosphorylation by a small interfering peptide, siLIP, promotes the synaptic localization of PSD-95 and enhances synaptic strength in vivo. Our findings collectively demonstrate that the Cdk5-dependent phosphorylation of liprin alpha 1 is important for the postsynaptic organization during activity-dependent synapse development.
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