DNA methylation of intragenic CpG islands depends on their transcriptional activity during differentiation and disease

The human genome contains similar to 30,000 CpG islands (CGIs). While CGIs associated with promoters nearly always remain unmethylated, many of the similar to 9,000 CGIs lying within gene bodies become methylated during development and differentiation. Both promoter and intragenic CGIs may also become abnormally methylated as a result of genome rear-rangements and in malignancy. The epigenetic mechanisms by which some CGIs become methylated but others, in the same cell, remain unmethylated in these situations are poorly understood. Analyzing specific loci and using a genome-wide analysis, we show that transcription running across CGIs, associated with specific chromatin modifications, is required for DNA methyltransferase 3B (DNMT3B)-mediated DNA methylation of many naturally occurring intragenic CGIs. Importantly, we also show that a subgroup of intragenic CGIs is not sensitive to this process of transcription -mediated methylation and that this correlates with their individual intrinsic capacity to initiate transcription in vivo. We propose a general model of how transcription could ad as a primary determinant of the patterns of CGI methylation in normal development and differentiation, and in human disease.