Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 114, Issue 7, Pages E1282-E1290Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1610212114
Keywords
positive-strand RNA virus; viral RNA replication; reactive oxygen species; respiratory burst oxidase homolog; calcium-dependent protein kinase
Categories
Funding
- Okayama University Startup fund
- Japan Society for the Promotion of Science (JSPS) KAKENHI Grant [15H06420]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [16H06436]
- JSPS KAKENHI [22248002, 15H04456]
- Grants-in-Aid for Scientific Research [15K14681, 16H06436, 15H06420, 15H04456, 26111008, 16H06429, 25252011, 16H01207, 22248002, 15H01239, 26292032, 17H01463] Funding Source: KAKEN
Ask authors/readers for more resources
As sessile organisms, plants have to accommodate to rapid changes in their surrounding environment. Reactive oxygen species (ROS) act as signaling molecules to transduce biotic and abiotic stimuli into plant stress adaptations. It is established that a respiratory burst oxidase homolog B of Nicotiana benthamiana (NbRBOHB) produces ROS in response to microbe-associated molecular patterns to inhibit pathogen infection. Plant viruses are also known as causative agents of ROS induction in infected plants; however, the function of ROS in plant-virus interactions remains obscure. Here, we show that the replication of red clover necrotic mosaic virus (RCNMV), a plant positive-strand RNA [(+) RNA] virus, requires NbRBOHB-mediated ROS production. The RCNMV replication protein p27 plays a pivotal role in this process, redirecting the subcellular localization of NbRBOHB and a subgroup II calcium-dependent protein kinase of N. benthamiana (NbCDPKiso2) from the plasma membrane to the p27-containing intracellular aggregate structures. p27 also induces an intracellular ROS burst in an RBOH-dependent manner. NbCDPKiso2 was shown to be an activator of the p27-triggered ROS accumulations and to be required for RCNMV replication. Importantly, this RBOH-derived ROS is essential for robust viral RNA replication. The need for RBOHderived ROS was demonstrated for the replication of another (+) RNA virus, brome mosaic virus, suggesting that this characteristic is true for plant (+) RNA viruses. Collectively, our findings revealed a hitherto unknown viral strategy whereby the host ROS-generating machinery is diverted for robust viral RNA replication.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available