Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 114, Issue 29, Pages 7719-7724Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1618656114
Keywords
LGI1; Kv1 channels; D-type current; intrinsic excitability; epilepsy
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Funding
- Agence Nationale de la Recherche [ANR-11-BSV4-0016]
- Fondation pour la Recherche Medicale
- Spanish Government (Ministerio de Economia y Competitividad) [SAF2015-65315-R]
- Agence Nationale de la Recherche (ANR) [ANR-11-BSV4-0016] Funding Source: Agence Nationale de la Recherche (ANR)
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Autosomal dominant epilepsy with auditory features results from mutations in leucine-rich glioma-inactivated 1 (LGI1), a soluble glycoprotein secreted by neurons. Animal models of LGI1 depletion display spontaneous seizures, however, the function of LGI1 and the mechanisms by which deficiency leads to epilepsy are unknown. We investigated the effects of pure recombinant LGI1 and genetic depletion on intrinsic excitability, in the absence of synaptic input, in hippocampal CA3 neurons, a classical focus for epileptogenesis. Our data indicate that LGI1 is expressed at the axonal initial segment and regulates action potential firing by setting the density of the axonal Kv1.1 channels that underlie dendrotoxin-sensitive D-type potassium current. LGI1 deficiency incurs a >50% down-regulation of the expression of Kv1.1 and Kv1.2 via a posttranscriptional mechanism, resulting in a reduction in the capacity of axonal D-type current to limit glutamate release, thus contributing to epileptogenesis.
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