Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 114, Issue 23, Pages E4574-E4581Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1703057114
Keywords
vimentin; Notch; angiogenesis; Jagged
Categories
Funding
- Academy of Finland [218062]
- Sigrid Juselius Foundation
- Cancer Society of Finland
- Marie Curie CIG grant
- Graduate Programme of Molecular Biosciences at Abo Akademi University
- Magnus Ehrnrooth Foundation
- Swedish Cultural Foundation of Finland
- Waldemar von Frenckell Foundation
- Svensk-Osterbottniska Samfundet
- Finnish Cultural Foundation
- K. Albin Johansson Foundation
- Aarne Koskelo Foundation
- Einar and Karin Stroem Foundation
- Swedish Research Council
- European Union
- National Heart, Lung, and Blood Institute, NIH [R01HL095786]
- Academy of Finland (AKA) [218062, 218062] Funding Source: Academy of Finland (AKA)
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Notch signaling is a key regulator of angiogenesis, in which sprouting is regulated by an equilibrium between inhibitory Dll4-Notch signaling and promoting Jagged-Notch signaling. Whereas Fringe proteins modify Notch receptors and strengthen their activation by Dll4 ligands, other mechanisms balancing Jagged and Dll4 signaling are yet to be described. The intermediate filament protein vimentin, which has been previously shown to affect vascular integrity and regenerative signaling, is here shown to regulate ligand-specific Notch signaling. Vimentin interacts with Jagged, impedes basal recycling endocytosis of ligands, but is required for efficient receptor ligand transendocytosis and Notch activation upon receptor binding. Analyses of Notch signal activation by using chimeric ligands with swapped intracellular domains (ICDs), demonstrated that the Jagged ICD binds to vimentin and contributes to signaling strength. Vimentin also suppresses expression of Fringe proteins, whereas depletion of vimentin enhances Fringe levels to promote Dll4 signaling. In line with these data, the vasculature in vimentin knockout (VimKO) embryos and placental tissue is underdeveloped with reduced branching. Disrupted angiogenesis in aortic rings from VimKO mice and in endothelial 3D sprouting assays can be rescued by reactivating Notch signaling by recombinant Jagged ligands. Taken together, we reveal a function of vimentin and demonstrate that vimentin regulates Notch ligand signaling activities during angiogenesis.
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