Journal
SEPARATION SCIENCE PLUS
Volume 1, Issue 6, Pages 404-410Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/sscp.201800038
Keywords
arginine; dimethylarginine; homoeysteine; plasma; serum
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Funding
- CSIR under XII plan project [BSC0202]
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Elevated concentrations of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are considered as common risk factors for cardiovascular diseases. A simple reversed-phase high-performance liquid chromatography method has been developed to simultaneously detect these biomarkers in plasma and serum. Straightforward sample preparation involves direct derivatization of deproteinized serum/plasma with phenyl isothiocyanate. Dimethylarginines and homocysteine are resolved using a 250 x 4.6 mm I.D, C-18 column and a linear gradient of sodium acetate buffer/acetonitrile. Calibration curves of peak area verses concentration are linear with correlation coefficients near unity. The intraday and interday precisions measured by coefficient of variation of peak areas were 1.34-9.7, 2.7-6.7 and 0.3-7.1 for asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine, respectively. The recoveries of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are above 86%. Minimal sample preparation, good recoveries and fast run time makes this method suitable for the analysis of plasma and serum.
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