Journal
ENVIRONMENTAL CHEMISTRY LETTERS
Volume 13, Issue 1, Pages 117-123Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10311-015-0493-7
Keywords
Alkylsulfatase; Biosurfactant; Methylene blue active substance assay; Sodium dodecyl sulfate; Biodegradation
Funding
- Kerala State Council for Science, Technology and Environment (KSCSTE)
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Microbial remediation is commonly used to treat environmental samples polluted by anionic surfactants. However, this method is often limited due to the pathogenicity of the microbes used to clean environmental samples. Therefore, we aimed to isolate nonpathogenic bacterial strains capable of degrading sodium dodecyl sulfate. We used conventional enrichment culture. Strains were screened by methylene blue active substance assay and HPLC. The primary sodium dodecyl sulfate splitting enzyme alkylsulfatase was isolated by column chromatography. The enzymatic conversion of sodium dodecyl sulfate to dodecanol was followed by zymogram analysis. The biosurfactant producing ability of the isolate was measured using Siegmund Wagner blue agar, and the emulsification index was measured. The isolate was identified as Pseudomonas plecoglossicida S5 (JN700182), based on biochemical analysis and 16S rRNA sequencing. This strain S5 was able to degrade 89.28 +/- A 0.56 % of sodium dodecyl sulfate at 10 g/L. This strain produced a single type of alkylsulfatase enzyme and rhamnolipid biosurfactant in response to sodium dodecyl sulfate. Moreover, a 20 day fish study proved the nonpathogenicity of S5. This is the first report of sodium dodecyl sulfate degradation by P. plecoglossicida.
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