Journal
MIDDLE EAST FERTILITY SOCIETY JOURNAL
Volume 23, Issue 2, Pages 107-111Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mefs.2017.09.004
Keywords
Sertoli; Spermatogonial stem cells; Proliferation; Co-culture
Categories
Funding
- Mazandaran University of Medical Sciences, Sari, Iran [1365]
- Iranian Ministry of health and medical education
Ask authors/readers for more resources
Objectives: Sertoli cells effect the fate map of spermatogonial stem cells (SSCs) to self-renew via providing the special microenvironments. Maintenance of proliferation and self-renewal activity of SSCs may be usable as a therapeutic strategy, leads to increase the recovery of male fertility. This research was aimed to evaluate the effect of mouse sertoli cells on spermatogonia stem cells proliferation and the expression pattern of stemness markers. Methods: Spermatogonia stem cells were collected from neonatal mouse testis using a two-step mechanical and enzymatic digestion. SSCs were cultured in three groups: The first group or co-culture group consists of spermatogonia and sertoli cells that were cultured together. The control group, only spermatogonial cells and the group no. 3 included spermatogonial cells in the presence of GDNF. The colony formation of mentioned groups, was monitored during one month in culture. Identification of the colonies, was confirmed using PLZF and Oct4 immunostaining. Spermatogonial stemness genes includes; Stra8, mvh and piwill2 were analyzed by RT-PCR. Results: In the co-culture group, cells proliferated rapidly and many colonies were appeared whereas they were rarely formed in the control groups. Colonies were exhibited alkaline phosphatesase activity and were immunopositive to Oct4 and PLZF, strongly. The gene expression of srta8, mvh and piwill2, in SSCs that were cultivated with sertoli cells, were greater significantly than other control groups. Conclusion: It is concluded that co-culture of SSCs with sertoli cells prepares conditions which leads to efficient proliferation and maintenance of stemness condition of SSCs, that is usable as a therapeutic approach for treatment of male fertility. (C) 2017 Middle East Fertility Society. Production and hosting by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available