Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 47, Issue 7, Pages 730-738Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2017.1315597
Keywords
Amycolatopsis; biodegradation; poly(L-lactic acid); protease; response surface methodology; silk cocoon
Categories
Funding
- Royal Golden Jubilee Ph.D. Program [PHD/0142/2553]
- Center of Excellence on Biodiversity [BDC-PG2-159012]
- National Research University Project under Thailand's Office of the Higher Education Commission
- Graduate School, Chiang Mai University
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This study aims to find the optimal medium and conditions for polylactic acid (PLA)-degrading enzyme production by Amycolatopsis sp. SCM_MK2-4. Screening of the most effective components in the enzyme production medium by Plackett-Burman design revealed that the silk cocoon and PLA film were the most significant variables enhancing the PLA-degrading enzyme production. After an response surface methodology, a maximum amount of PLA-degrading enzyme activity at 0.74UmL(-1) was predicted and successfully validated at 95% after 0.39% (w/v) silk cocoon and 1.62% (w/v) PLA film were applied to the basal medium. The optimal initial pH value, temperature, and inoculum size were evaluated by a method considering one-factor-at-a-time. The values were recorded at an initial pH in the range of 7.5-9.0, a temperature of 30-32 degrees C, and an inoculum size of 4-10%. The highest activity of approximately 0.95UmL(-1) was achieved after 4 days of cultivation using the optimized medium and under optimized conditions in a shake flask. Upscaling to the use of a 3-L stirred tank fermenter was found to be successful with a PLA-degrading activity of 5.53UmL(-1); which represents a 51-fold increase in the activity compared with that obtained from the nonoptimized medium and conditions in the shake flask.
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