Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 47, Issue 5, Pages 520-529Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2016.1275009
Keywords
Cellooligosaccharides; cellulase; cellulose hydrolysis; controlled depolymerization; differential adsorption; exocellulases
Categories
Funding
- Department of Biotechnology, India
- University Grant Commission, India
Ask authors/readers for more resources
Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose. Preferential adsorption of exocellulases was observed over endocellulase, while -glucosidases remained unadsorbed. Adsorbed enzyme fraction with bound exocellulases when used for hydrolysis generated cellobiose predominantly, while the unadsorbed enzymes in the liquid fraction produced cellooligosaccharides majorly, owing to its high endocellulases activity. Thus, the differential adsorption phenomenon of the cellulase components can be used for the controlling cellulose hydrolysis for the production of an array of sugars.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available