Journal
PHYSICAL BIOLOGY
Volume 15, Issue 5, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/1478-3975/aac008
Keywords
cell kinematics; human embryonic stem cells; cell migration; correlated random walk
Categories
Funding
- Newcastle University
- European Community (IMI-STEMBANCC, IMI-EBISC) [614620, NC3R NC/CO16206/1]
- School of Mathematics, Statistics and Physics of Newcastle University
- Leverhulme Trust [RPG-2014-427]
- MRC [G0301182] Funding Source: UKRI
Ask authors/readers for more resources
We perform a detailed analysis of the migratory motion of human embryonic stem cells in twodimensions, both when isolated and in close proximity to another cell, recorded with time-lapse microscopic imaging. We show that isolated cells tend to perform an unusual locally anisotropic walk, moving backwards and forwards along a preferred local direction correlated over a timescale of around 50 min and aligned with the axis of the cell elongation. Increasing elongation of the cell shape is associated with increased instantaneous migration speed. We also show that two cells in close proximity tend to move in the same direction, with the average separation of 70 mu m or less and the correlation length of around 25 mu m, a typical cell diameter. These results can be used as a basis for the mathematical modelling of the formation of clonal hESC colonies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available