Platinum aptasensor wire arrays for cardiac biomarker detection

There is clinical diagnostic power in developing point-of-care diagnostic biosensors offering rapid and sensitive detection of cardiac biomarkers. This could provide outpatient screening and monitoring of cardiovascular diseases in outpatient settings via portable technology. This study focuses on development of platinum wire platforms functionalized with aptamers to impedimetrically detect Brain Natriuretic Peptide (BNP) and Troponin T (TnT), biomarkers, the currently accepted gold standard indicators of congestive heart failure and myocardial infarction. Various platinum wire diameters and surface preparation conditions were selected to determine ideal electrode parameters for impedimetric detection of BNP and TnT without loss of precision and sensitivity. Ideal parameters of 0.5 mm diameter wire polished to 5 mu m demonstrated optimal linearity, correlation, and precision when detecting BNP and TnT. The authors have thus developed a simplistic platinum wire multi-array apatasensor platform for detecting BNP and TnT easily expandable to detecting various biomarkers in portable or handheld POC biosensing device configurations.