In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B-1 (5 mu g/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B-1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B-1 and hydrolysed fumonisin B-1 concentration were analysed by liquid chromatograghy - mass spectrometry. There was no significant difference in FB1, concentration between the experimental and the control 2 group (buffer and fumonisin B-1) at Oh incubation, 5.185 +/- 0.174 mu g/ml compared with 6.43 +/- 30.076 mu g/ml. Fumonisin B-1 concentration in the experimental group was reduced to 4.080 +/- 0.065 mu g/m1 at 24 h and to 2.747 +/- 0.548 mu g/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B-1 was detected after 24 h incubation (0.012 +/- 0 mu g/m1). At 48 h incubation time, hydrolysed fumonisin B-1 concentration was doubled to 0.024 +/- 0.004 mu g/ml. These results indicate that fumonisin B-1 can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.