Cloning of acetyl CoA carboxylase cDNA and the effects of spirodiclofen on the expression of acetyl CoA carboxylase mRNA in Panonychus citri

The citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), is an important pest worldwide. Previous studies showed that acetyl CoA carboxylase (ACCase) may be a critical target of spirodiclofen, a recently introduced insecticide. Therefore, we cloned the PcACCase gene and obtained a full-length cDNA (Genbank accession number ). The open reading frame was 6972bp, coding for 2323 amino acids. The PcACCase protein had a theoretical molecular weight of 262.82kDa and an isoelectric point of 6.31, and it contained six conserved domains and one low-complexity region. Quantitative real-time PCR showed that spirodiclofen can up-regulate the expression levels of ACCase mRNA during all four developmental stages.