Journal
PLOS ONE
Volume 12, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0190202
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Funding
- National Natural Science Foundation of China [31401329]
- China Postdoctoral Science Foundation [2014M560724, 2017T100707]
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In this study, the extraction of water-soluble polysaccharides from the seed coat of black soybean (BSCP) was investigated and optimized. A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction temperature, 100 degrees C;ratio of water to material, 22.3 mL/g; and extraction time, 133.2 min. Under these conditions, the experimental yield of polysaccharides was 10.56%, which was consistent with the predictive yield. A novel galactomannan, BSCP-1, with a molecular weight of 7.55 x 10(5) Da determined by high-performance gel permeation chromatography, was isolated from the black soybean seed coat. Through gas chromatographymass spectrometry analysis, BSCP-1 was identified as a galactomannan consisting of galactose, mannose and rhamnose in a molar ratio of 6.01: 3.56: 1.00. Cytotoxicity against the human gastric carcinoma cancer cell line was also determined.
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