3.8 Article

HYDROTROPE-INDUCED MICELLAR PHASE OF SODIUM DODECYL SULFATE AS NEW DETERGENT FOR EXTRACTION AND STABILIZATION OF PROTEINS

Journal

CHEMISTRY & CHEMICAL TECHNOLOGY
Volume 12, Issue 2, Pages 196-201

Publisher

LVIV POLYTECH NATL UNIV
DOI: 10.23939/chcht12.02.196

Keywords

micellar extraction; sodium dodecyl sulfate; protein; native state; catalytic activity

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X-ray phase analysis and IR spectroscopy data have confirmed a gel-like nature of sodium dodecyl sulfate micellar phase induced by sodium chloride and salicylic acid additives. This phase essentially consists of water (omega(H2O) > 50 %), which is connected with main components of the micellar-extraction system through intermolecular hydrogen bonds. Capacity of such micellar phase to quantitatively extract of proteins (R > 94 %) with saving of their catalytic activities and dispersity was found.

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