Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 111, Issue -, Pages 30-38Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2016.11.009
Keywords
Wheat; Metabolomics; Low nitrogen stress; UPLC-QTOF; PCA; OPLS-DA
Categories
Funding
- National Natural Science Founds of China [30271650, 31301281]
- State Key Laboratory of Wheat and Maize Crop Science in China
- Special Industry Scientific Research Funds [201303109-6]
Ask authors/readers for more resources
Wheat is one of the most important grain crop plants worldwide. Nitrogen (N) is an essential macro nutrient for the growth and development of wheat and exerts a marked influence on its metabolites. To investigate the influence of low nitrogen stress on various metabolites of the flag leaf of wheat (Triticum aestivum L.), a metabolomic analysis of two wheat cultivars under different induced nitrogen levels was conducted during two important growth periods based on large-scale untargeted metabolomic analysis using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLCQTOF). Multivariate analyses such as principle components analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) were used for data analysis. PCA yielded distinctive clustering information among the samples, classifying the wheat flag samples into two categories: those under normal N treatment and low N treatment. By processing OPLS-DA, eleven secondary metabolites were shown to be responsible for classifying the two groups. The secondary metabolites may be considered potential biomarkers of low nitrogen stress. Chemical analyses showed that most of the identified secondary metabolites were flavonoids and their related derivatives, such as iso-vitexin, iso-orientin and methylisoorientin-2-O-rhamnoside, etc. This study confirmed the effect of low nitrogen stress on the metabolism of wheat, and revealed that the accumulation of secondary metabolites is a response to abiotic stresses. Meanwhile, we aimed to identify markers which could be used to monitor the nitrogen status of wheat crops, presumably to guide appropriate fertilization regimens. Furthermore, the UPLC-QTOF metabolic platform technology can be used to study metabolomic variations of wheat under abiotic stresses. (C) 2016 Elsevier Masson SAS. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available