Superoxide-responsive gene expression in Arabidopsis thaliana and Zea mays

Superoxide (Or) and other reactive oxygen species (ROS) are generated in response to numerous biotic and abiotic stresses. Different ROS have been reported to elicit different transcriptional responses in plants, and so ROS-responsive marker genes and promoter::reporter gene fusions have been proposed as indirect means of detecting ROS and discriminating among different species. However, further information about the specificity of transcriptional responses to O-2(-) is needed in order to assess potential markers for this critical stress-responsive signaling molecule. Using qRT-PCR, the expression of 12 genes previously reported to be upregulated by O-2(-) was measured in Arabidopsis thaliana plants exposed to elicitors of common stress-responsive ROS: methyl viologen (an inducer of O-2(-)), rose bengal (an inducer of singlet oxygen, (1)Delta O-2), and exogenous hydrogen peroxide (H2O2). Surprisingly, Zinc-Finger Protein 12 (AtZAT12), which had previously been used as a reporter for H2O2, responded more strongly to O-2(-) than to H2O2; moreover, the expression of an AtZAT12 promoter-reporter fusion (AtZAT12::Luc) was enhanced by diethyldithiocarbamate, which inhibits dismutation of O-2(-) to H2O2. These results suggest that AtZAT12 is transcriptionally upregulated in response to of, and that AtZAT12::Luc may be a useful biosensor for detecting O-2(-) generation in vivo. In addition, transcripts encoding uncoupling proteins (AtUCPs) showed selectivity for O-2(-) in Arabidopsis, and an AtUCP homolog upregulated by methyl viologen was also identified in maize (Zea mays L), indicating that there are O-2(-)- responsive members of this family in monocots. These results expand our limited knowledge of ROS-responsive gene expression in monocots, as well as O-2(-)-selective responses in dicots. (C) 2017 The Authors. Published by Elsevier Masson SAS.