Journal
PLANT PHYSIOLOGY
Volume 175, Issue 3, Pages 1094-1104Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.17.00737
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Funding
- Max Planck Society
- Fonds der Chemischen Industrie
- German Research Foundation (DFG) [PF850/1-1]
- Carlsberg Foundation
- Danish Strategic Research Council (GlycAct project)
- Danish Strategic Research Council (SET4Future project)
- Villum Foundation (PLANET)
- U.S. Natural Science Foundation [DBI-0421683, IOS-0923992]
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In the last three decades, more than 200 monoclonal antibodies have been raised against most classes of plant cell wall polysaccharides by different laboratories worldwide. These antibodies are widely used to identify differences in plant cell wall components in mutants, organ and tissue types, and developmental stages. Despite their importance and broad use, the precise binding epitope has been determined for only a few of these antibodies. Here, we use a plant glycan microarray equipped with 88 synthetic oligosaccharides to comprehensively map the epitopes of plant cell wall glycan-directed antibodies. Our results reveal the binding epitopes for 78 arabinogalactan-, rhamnogalacturonan-, xylan-, and xyloglucan-directed antibodies. We demonstrate that, with knowledge of the exact epitopes recognized by individual antibodies, specific glycosyl hydrolases can be implemented into immunological cell wall analyses, providing a framework to obtain structural information on plant cell wall glycans with unprecedented molecular precision.
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