Journal
PLANT JOURNAL
Volume 92, Issue 4, Pages 638-649Publisher
WILEY
DOI: 10.1111/tpj.13709
Keywords
organelle RNA editing; mitochondrion; pentatricopeptide repeat; DYW domain; deaminase; Arabidopsis thaliana
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Funding
- National Science Foundation [MCB-0929423, MCB-1615393]
- National Science Foundation Graduate Research Fellowship [DGE-1321846]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1615393] Funding Source: National Science Foundation
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An RNA-seq approach was used to investigate the role of a PLS-subfamily pentatricopeptide repeat protein, Mitochondrial Editing Factor 8 (MEF8), on editing in Arabidopsis mitochondria and plastids. MEF8 has an intact DYW domain, but possesses an unusually short PLS repeat region of only five repeats. The MEF8 T-DNA insertion (mef8) line exhibited reduced editing at 38 mitochondrial editing sites and increased editing at 24 sites; therefore the absence of MEF8 affects 11% of the mitochondrial editome. Notably, 60% of the matR transcripts' sites showed a decrease of editing extent in the mef8 mutant. An E549A substitution in the MEF8 protein replaced the putatively catalytic glutamate of the HXE motif in the DYW domain. Complementation with MEF8-E549A failed to restore editing at the main target sites but was able to restore editing at the matR transcript; it also decreased the editing extent of most of the C targets exhibiting an increase of editing extent in the mef8 mutant plant. Thus, MEF8 has two antagonistic effects on mitochondrial editing: stimulatory, which requires a catalytic glutamate for most of the targets except for the matR transcript, and inhibitory, for which glutamate is dispensable. Significance Statement MEF8 is a pentatricopeptide repeat protein that is required for C-to-U editing in the mitochondria of Arabidopsis plants. Analysis of mef8 null mutants and of transgenic plants based on next generation sequencing demonstrates that the MEF8 protein plays antagonistic roles in mitochondrial editing for which a glutamate residue is required or not.
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