Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 130, Issue 3, Pages 469-481Publisher
SPRINGER
DOI: 10.1007/s11240-017-1226-3
Keywords
Betula platyphylla Suk.; SPL gene family; Bioinformatics; Gene expression; Transgenic; Abiotic stress response
Funding
- Fundamental Research Funds for the Central Universities [DL12CA04]
- 111 Project [B16010]
- National Science and Technology Program of China [2013AA102704]
- China Postdoctoral Science Foundation [2014M560275]
- Open Foundation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [201203]
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SQUAMOSA promoter binding protein-like (SPL) genes encode plant-specific transcription factors and play vital roles in plant growth and development. The BpSPL gene family has not been systematically studied in Betula platyphylla Suk. (birch). In this study, we identified 12 full-length BpSPLs and analyzed their gene structure and distribution patterns in the birch genome. The phylogenetic analysis of SPLs in different plants species revealed six groups. Furthermore, seven of the 12 BpSPLs contained miR156 recognition sites. Comparative analysis of the spatiotemporal expression patterns of BpSPLs and miR156 uncovered an inverse relationship, suggesting a regulatory role for miR156-SPL circuits. We showed that the expression of BpSPL9 was induced by NaCl and PEG6000 stresses in roots and leaves. Through transgenic experiments, we obtained 18 independent lines with ectopic expression of BpSPL9. Moreover, physiological and enzymatic analyses of transgenic lines revealed improved scavenging of ROS to salt and drought stress. Our results will provide useful information for elucidating the biological functions of SPLs in B. platyphylla with potential applications in improving scavenging of ROS.
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