Journal
PLANT CELL AND ENVIRONMENT
Volume 40, Issue 5, Pages 658-671Publisher
WILEY
DOI: 10.1111/pce.12883
Keywords
HKT1; 2; post-transcriptional gene silencing; Solanum lycopersicum and Solanum cheesmaniae
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Funding
- ERDF from Spanish Ministerio de Economia, Industria y Competitividad' [AGL2010-17090, AGL2013-41733-R, AGL2015-64991-C3-3-R, AGL2014-56675-R]
- Proyecto de Excelencia, from Junta de Andalucia [CVI-7558]
- Australian Research Council (ARC) for Centre of Excellence [CE14010008]
- Future Fellowship [FT130100709]
- FPI program [BES-2011-046096]
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Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+/K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato.
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