Journal
PLANT CELL
Volume 29, Issue 9, Pages 2214-2232Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.17.00180
Keywords
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Funding
- National Natural Science Foundation of China [31630062, 31471746]
- National Program on Key Basic Research Project of China (973 Program) [2014CB138400]
- Youth Talent Support Program of China
- Distinguished Professor of Jiangsu Province
- Special Fund for Agro-Scientific Research in the Public Interest [201303028]
- National Science Foundation [NSF-IOS-1354434, NSF-IOS-1339185]
- Direct For Biological Sciences [1339185] Funding Source: National Science Foundation
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Plants use both cell surface-resident pattern recognition receptors (PRRs) and intracellular nucleotide binding leucine-rich repeat (NLR) receptors to detect various pathogens. Plant PRRs typically recognize conserved pathogen-associated molecular patterns (PAMPs) to provide broad-spectrum resistance. By contrast, plant NLRs generally detect pathogen strain-specific effectors and confer race-specific resistance. Here, we demonstrate that the tomato (Solanum lycopersicum) NLR Sw-5b confers broad-spectrum resistance against American-type tospoviruses by recognizing a conserved 21-amino acid peptide region within viral movement protein NSm (NSm21). Sw-5b NB-ARC-LRR domains directly associate with NSm21 in vitro and in planta. Domain swap, site-directed mutagenesis and structure modeling analyses identified four polymorphic sites in the Sw-5b LRR domain that are critical for the recognition of NSm21. Furthermore, recognition of NSm21 by Sw-5b likely disturbs the residues adjacent to R927 in the LRR domain to weaken the intramolecular interaction between LRR and NB-ARC domains, thus translating recognition of NSm21 into activation of Sw-5b. Natural variation analysis of Sw-5b homologs from wild tomato species of South America revealed that the four polymorphic sites in the Sw-5b LRR domain were positively selected during evolution and are all necessary to confer resistance to tospovirus. The results described here provide a new example of a plant NLR mediating broad-spectrum resistance through recognition of a small conserved PAMP-like region within the pathogen effector.
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