Journal
PLANT CELL
Volume 29, Issue 8, Pages 1883-1906Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.16.00953
Keywords
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Funding
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB11030200]
- National Key Research and Development Program of China [2016YFD0100603-10]
- Ministry of Agriculture of the People's Republic of China (Chinese Ministry of Agriculture) [2016ZX08009-003-001]
- National Natural Science Foundation of China [31030006, 31672157, 31390423]
- Tai-Shan Scholar Program from Shandong Province
- Hong Kong UGC [14104515/ 14119814]
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The hormone jasmonate (JA), which functions in plant immunity, regulates resistance to pathogen infection and insect attack through triggering genome-wide transcriptional reprogramming in plants. We show that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato (Solanum lycopersicum) acts downstream of the JA receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses. Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays, we identified 655 MYC2-targeted JA-responsive genes. These genes are highly enriched in Gene Ontology categories related to TFs and the early response to JA, indicating that MYC2 functions at a high hierarchical level to regulate JA-mediated gene transcription. We also identified a group of MYC2-targeted TFs (MTFs) that may directly regulate the JA-induced transcription of late defense genes. Our findings suggest that MYC2 and its downstream MTFs form a hierarchical transcriptional cascade during JA-mediated plant immunity that initiates and amplifies transcriptional output. As proof of concept, we showed that during plant resistance to the necrotrophic pathogen Botrytis cinerea, MYC2 and the MTF JA2-Like form a transcription module that preferentially regulates wounding-responsive genes, whereas MYC2 and the MTF ETHYLENE RESPONSE FACTOR. C3 form a transcription module that preferentially regulates pathogen-responsive genes.
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