Journal
PLANT BREEDING
Volume 136, Issue 1, Pages 50-60Publisher
WILEY
DOI: 10.1111/pbr.12445
Keywords
maize; haploid; diploid; fluorescence; automated sorting
Funding
- EU [324433]
- Iowa State University Raymond F. Baker Center for Plant Breeding
Ask authors/readers for more resources
The use of doubled haploids (DHs) in maize has become ubiquitous in maize breeding programmes as it allows breeders to go from cross to evaluation in as little as 2years. Two important aspects of the invivo DH system used in maize are as follows: (i) the identification of haploid progeny and (ii) doubling of the haploid genome to produce fertile inbred lines. This study is focused on the first step. Currently, identification of maize haploid progeny is performed manually using the R1-nj seed colour marker. This is a labour-intensive and time-consuming process; a method for automated sorting of haploids would increase the efficiency of DH line development. In this study, six inbred lines were crossed with the maternal haploid inducer RWS/RWK-76' and a sample of seed was sorted manually for each line. Using the VideometerLab 3 system, spectral imaging techniques were applied to discriminate between haploids and hybrids. Using DNA markers to confirm the haploid/diploid state of the tested seed, for the majority of genotypes haploid identification was possible with over 50% accuracy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available