Journal
PLANT BIOTECHNOLOGY REPORTS
Volume 11, Issue 3, Pages 147-160Publisher
SPRINGER
DOI: 10.1007/s11816-017-0437-8
Keywords
Xanthine dehydrogenase; Arabidopsis; Transgenic plants; Salt tolerance
Funding
- Shanghai Municipal Committee of Agriculture [zhongzi2013-8, zhongzi2014-2, gongzi2014-7-3, zhongzi2016-1-2]
- Agriculture science technology achievement transformation fund [143919N0300]
- Basic research in the field of science and technology project of Science and Technology Commission of Shanghai Municipality [14JC1403602]
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Xanthine dehydrogenase (EC1.1.1.204; XDH) plays an important role in purine catabolism that catalyzes the oxidative hydroxylation of hypoxanthine to xanthine and of xanthine to uric acid. Long attributed to its role in recycling and remobilization of nitrogen, recently, XDH is implicated in plant stress responses and acclimation, such research efforts, however, have thus far been restricted to Arabidopsis XDH-knockdown/knockout studies. This study, using an ectopic overexpression approach, is expected to provide novel findings. In this study, a XDH gene from Vitis vinifera, named VvXDH, was synthesized and overexpressed in Arabidopsis, the transgenic Arabidopsis showed enhanced salt tolerance. The VvXDH gene was investigated and the results demonstrated the explicit role of VvXDH in conferring salt stress by increasing allantoin accumulation and activating ABA signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis. In addition, the water loss and chlorophyll content loss were reduced in transgenic plants; the transgenic plants showed higher proline level and lower MDA content than that of wild-type Arabidopsis, respectively. In conclusion, the VvXDH gene has the potential to be applied in increasing allantoin accumulation and enhancing the tolerance to abiotic stresses in Arabidopsis and other plants.
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