Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 16, Issue 6, Pages 1227-1240Publisher
WILEY
DOI: 10.1111/pbi.12865
Keywords
3D protein modelling; abiotic stress; activation domain; phenotypic features; protein homo-and hetero-dimerization; stress-inducible promoters
Funding
- University of Adelaide
- Australian Research Council Industrial Transforming Research Hub [IH130200027]
- Dupont-Pioneer
- Australian Research Council Linkage Project [LP120100201]
- Australian Grains Research and Development Corporation
- Government of South Australia
- BBSRC [BBS/E/C/000I0310] Funding Source: UKRI
- Australian Research Council [IH130200027] Funding Source: Australian Research Council
Ask authors/readers for more resources
Characterization of the function of stress-related genes helps to understand the mechanisms of plant responses to environmental conditions. The findings of this work defined the role of the wheat TaHDZipI-5 gene, encoding a stress-responsive homeodomain-leucine zipper class I (HDZip I) transcription factor, during the development of plant tolerance to frost and drought. Strong induction of TaHDZipI-5 expression by low temperatures, and the elevated TaHDZipI-5 levels of expression in flowers and early developing grains in the absence of stress, suggests that TaHDZipI-5 is involved in the regulation of frost tolerance at flowering. The TaHDZipI-5 protein behaved as an activator in a yeast transactivation assay, and the TaHDZipI-5 activation domain was localized to its C-terminus. The TaHDZipI-5 protein homo-and hetero-dimerizes with related TaHDZipI-3, and differences between DNA interactions in both dimers were specified at 3D molecular levels. The constitutive overexpression of TaHDZipI-5 in bread wheat significantly enhanced frost and drought tolerance of transgenic wheat lines with the appearance of undesired phenotypic features, which included a reduced plant size and biomass, delayed flowering and a grain yield decrease. An attempt to improve the phenotype of transgenic wheat by the application of stress-inducible promoters with contrasting properties did not lead to the elimination of undesired phenotype, apparently due to strict spatial requirements for TaHDZipI-5 overexpression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available