Journal
PLANT BIOLOGY
Volume 19, Issue 4, Pages 608-617Publisher
WILEY
DOI: 10.1111/plb.12556
Keywords
Biological control; citrus canker disease; enantio-pyochelin; indigenous; lemon; Pseudomonas
Categories
Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [PICT 1939, PICT 2222]
- Consejo de Investigaciones de la UNT [CIUNT 26/D439]
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Citrus canker is a worldwide-distributed disease caused by Xanthomonas citri subsp. citri. One of the most used strategies to control the disease is centred on copper-based compounds that cause environmental problems. Therefore, it is of interest to develop new strategies to manage the disease. Previously, we reported the ability of the siderophore pyochelin, produced by the opportunistic human pathogen Pseudomonas aeruginosa, to inhibit invitro several bacterial species, including X.citri subsp. citri. The action mechanism, addressed with the model bacterium Escherichia coli, was connected to the generation of reactive oxygen species (ROS). This work aimed to find a non-pathogenic strain from the lemon phyllosphere that would produce pyochelin and therefore serve in canker biocontrol. An isolate that retained its capacity to colonise the lemon phyllosphere and inhibit X.citri subsp. citri was selected and characterised as Pseudomonas protegens CS1. From a liquid culture of this strain, the active compound was purified and identified as the pyochelin enantiomer, enantio-pyochelin. Using the producing strain and the pure compound, both invitro and invivo, we determined that the action mechanism of X.citri subsp. citri inhibition also involved the generation of ROS. Finally, the potential application of P.protegens CS1 was evaluated by spraying the bacterium in a model that mimics the natural X.citri subsp. citri infection. The ability of P.protegens CS1 to reduce canker formation makes this strain an interesting candidate as a biocontrol agent.
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