SP100B Expression Indexed Hemorrhage in Mouse Models of Cerebral Hemorrhage

The aim of the present study was to investigate the expression of SP100B in mouse models with acute cerebral hemorrhage. Mouse models of cerebral hemorrhage were induced using injection of collagenase into the brain. The serum levels of SP100B were detected by ELISA. The expression of SP100B in the hippocampus and other brain tissue were detected by indirect immunofluorescence technique. The mean concentration of serum SP100B was significantly higher in hemorrhage group (0.85 mu g/l) than in control group (0.20 mu g/l) (P = 0.0017). More importantly, the mean value of serum SP100B in both moderate hemorrhage (0.96 mu g/l) and severe hemorrhage (1.21 mu g/l) had significantly higher compared to hyporrhea group (0.39 mu g/l) (P = 0.0041 and P = 0.0009, respectively). The expression of SP100B also increased in the hippocampus with severe hemorrhage. Additionally, the expression of SP100B was high in the early stage of hemorrhage. SP100B expression was positively related to the severity of hemorrhage in mouse models of cerebral hemorrhage. Serum SP100B might be a noninvasive biomarker for cerebral hemorrhage.