Journal
PLANT AND CELL PHYSIOLOGY
Volume 58, Issue 12, Pages 2190-2201Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcx153
Keywords
Abscisic acid; Arabidopsis thaliana; Asymmetric leaves2-like; Lateral root; LBD14; Lateral organ boundaries domain; RNAi
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Funding
- Rural Development Administration, Republic of Korea [Next-Generation BioGreen 21 Program] [PJ01104701]
- Ministry of Education, Science, and Technology of Korea [Mid-career Researcher Program through the National Research Foundation of Korea] [2016R1A2B4015201]
- Ministry of Education, Science, and Technology of Korea [Basic Research Laboratory through the National Research Foundation of Korea] [2017R1A4A1015620]
- National Research Foundation of Korea [2017R1A4A1015620, 2016R1A2B4015201] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN/ASYMMETRIC LEAVES2-LIKE (LBD/ASL) gene family members play key roles in diverse aspects of plant development. Previous studies have shown that LBD16, 18, 29 and 33 are critical for integrating the plant hormone auxin to control lateral root development in Arabidopsis thaliana. In the present study, we show that LBD14 is expressed exclusively in the root where it promotes lateral root (LR) emergence. Repression of LBD14 expression by ABA correlates with the inhibitory effects of ABA on LR emergence. Transient gene expression assays with Arabidopsis protoplasts demonstrated that LBD14 is a nuclear-localized transcriptional activator. The knock-down of LBD14 expression by RNA interference (RNAi) resulted in reduced LR formation by delaying both LR primordium development and LR emergence, whereas overexpression of LBD14 in Arabidopsis enhances LR formation. We show that ABA (but not other plant hormones such as auxin, brassinosteroids and cytokinin) specifically downregulated b-glucuronidase (GUS) expression under the control of the LBD14 promoter in transgenic Arabidopsis during LR development from initiation to emergence and endogenous LBD14 transcript levels in the root. Moreover, RNAi of LBD14 enhanced the LR suppression in response to ABA, whereas LBD14 overexpression did not alter the ABAmediated suppression of LR formation. Taken together, these results suggest that LBD14 promoting LR formation is one of the critical factors regulated by ABA to inhibit LR growth, contributing to the regulation of the Arabidopsis root system architecture in response to ABA.
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