Journal
PLACENTA
Volume 51, Issue -, Pages 70-75Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2017.02.001
Keywords
microRNA-520g; Pre-eclampsia; Trophoblast; HTR8/SVneo; Matrix metalloproteinase 2; Biomarker
Funding
- Shanghai Municipal Science and Technology Commission in China [14411973600]
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Introduction: Pre-eclampsia (PE) is a common and severe obstetric complication. MicroRNAs (miRs) have emerged as molecules that are associated with the disease. Methods: Quantitative reverse transcription PCR (RT-qPCR) was used for serum miR-520g characterization from 19 severe pre-eclamptic and 19 normal pregnancies. In situ hybridation was adopted to localize ipicroRNA-520g (miR-520g). Migration and invasion of HTR-8/SVneo cells were evaluated after miR-520g mimic treatment with transwell system. MiR-520g target gene was verified in luciferase reporter system. Results: The expression of serum miR-520g displayed an upward trend as pregnancies progress. At first trimester, miR-520g in pre-eclampsia was significantly higher than that in the control, but no significant differences were found in the second and last trimesters. MiR-520g localized in cytoplasm of early trimester placental trophoblasts. The migration and invasion of HTR8/SVneo were inhibited by miR-520g mimic treatment. Matrix metalloproteinase 2 (MMP2) was verified as a direct target of miR-520g. Conclusions: Elevated maternal serum level of miR-520g level in first trimester was detected in patients with severe PE. By suppressing the migration and invasion of trophoblast via at least partial inhibition of MMP2 translation inhibition, miR-520g might play a role in the defective spiral artery remodeling, and thus contribute to pre-eclampsia pathophysiology. (C) 2017 Elsevier Ltd. All rights reserved.
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