Journal
PLACENTA
Volume 52, Issue -, Pages 17-20Publisher
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2017.02.010
Keywords
Trophoblast cell lines; Cellular immortalization; Telomerase; Karyotype; Polyploidy; Short tandem repeat profile
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Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103 similar to 119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69 similar to 81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells. (C) 2017 Elsevier Ltd. All rights reserved.
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