Journal
PIGMENT CELL & MELANOMA RESEARCH
Volume 30, Issue 6, Pages 531-540Publisher
WILEY
DOI: 10.1111/pcmr.12609
Keywords
cultured human melanocytes; engineered skin substitutes; photoprotection; skin pigmentation; wound closure
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Funding
- US Department of Defense
- Armed Forces Institute for Regenerative Medicine [W81XWH-13-2-0054]
- Shriners Hospitals for Children [84050]
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Autologous engineered skin substitutes (ESS) containing melanocytes (hM) may restore pigmentation and photoprotection after grafting to full-thickness skin wounds. In this study, normal hM were isolated from discard skin, propagated with or without tyrosinase inhibitors, cryopreserved, recovered into culture, and added to ESS (ESS-P) before transplantation. ESS-P were incubated in either UCMC160/161 or UCDM1 medium, scored for hM densities, and grafted to mice. The results showed that sufficient hM can be propagated to expand donor tissue by 100-fold; incubation of hM in tyrosinase inhibitors reduced pigment levels but did not change hM recovery after cryopreservation; hM densities in ESS-P were greater after incubation in UCDM1 than UCMC160 medium; hM were localized to the dermal-epidermal junction of ESS-P; and UCDM1 medium promoted earlier pigment distribution and density. These results indicate that hM can be incorporated into ESS-P efficiently to restore cutaneous pigmentation and UV photoprotection after full-thickness skin loss conditions.
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