Journal
PHYTOTHERAPY RESEARCH
Volume 31, Issue 4, Pages 680-688Publisher
WILEY
DOI: 10.1002/ptr.5791
Keywords
lung cancer stem cells; curcumin; Wnt/beta-catenin pathway; Sonic Hedgehog pathway; inhibition
Categories
Funding
- National Basic Research Program of China (973 Program) [2013CB910303]
- National Natural Science Foundation of China [81573139]
- Science and Technology Planning Project of Guangdong Province of China [2013B022000041]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Cancer stem cells (CSCs) are highly implicated in the progression of human cancers. Thus, targeting CSCs may be a promising strategy for cancer therapy. Wnt/beta-catenin and Sonic Hedgehog pathways play an important regulatory role in maintaining CSC characteristics. Natural compounds, such as curcumin, possess chemopreventive properties. However, the interventional effect of curcumin on lung CSCs has not been clarified. In the present study, tumorsphere formation assay was used to enrich lung CSCs from A549 and H1299 cells. We showed that the levels of lung CSC markers (CD133, CD44, ALDHA1, Nanog and Oct4) and the number of CD133-positive cells were significantly elevated in the sphere-forming cells. We further illustrated that curcumin efficiently abolished lung CSC traits, as evidenced by reduced tumorsphere formation, reduced number of CD133-positive cells, decreased expression levels of lung CSC markers, as well as proliferation inhibition and apoptosis induction. Moreover, we demonstrated that curcumin suppressed the activation of both Wnt/beta-catenin and Sonic Hedgehog pathways. Taken together, our data suggested that curcumin exhibited its interventional effect on lung CSCs via inhibition of Wnt/beta-catenin and Sonic Hedgehog pathways. These novel findings could provide new insights into the potential therapeutic application of curcumin in lung CSC elimination and cancer intervention. Copyright (C) 2017 John Wiley & Sons, Ltd.
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