Mannose binding lectin-2 gene functional polymorphisms in chronic periodontitis patients; a report from Iran

Chronic Periodontitis (CP)(1) is a bacterial inflammatory condition accompanied with destruction of tissues surrounding periodontal ligament and tooth loss. There are reports of associations between mannose binding lectin-2 (MBL2)(2) gene polymorphisms and susceptibility to bacterial infections, however, a few studies have been performed on the role of MBL polymorphisms in periodontal diseases. Here, we assessed the potential impacts of two functional polymorphisms in MBL2 gene in CP. Forty CP patients along with 50 healthy controls were included. Two polymorphisms of MBL2 gene (rs1800451 and rs7096206) were assessed by Tetra-ARMS (Amplification Refractory Mutation System) PCR. Heterozygous (AG) and variant homozygous (AA) genotypes of rs1800451 polymorphism were detected in 17 (42.5%) and 2 (5%) of CP patients, and 17 (34%), and 1 (2%) in controls respectively (P = 0.4). The variant allele frequency was 27.5% in CP patients and 20% in controls (P = 0.2). For the rs7096206 polymorphism, heterozygous (CG) genotype was identified in 22 (55%) and 19 (38%) in healthy subjects respectively (P = 0.1). Variant allele homozygous (GG) was only detected in one CP patient, and none of the healthy individuals. There was no significant difference in means of Clinical Attachment Loss(3) (CAL) and Pocket Depth(4) (PD) values regarding different genotypes. Although logistic regression analysis rendered odd ratio of 1.4 and 2.6, and 2 for AG and AA genotypes of rs1800451 polymorphism and CG genotype of rs7096206 polymorphism respectively, these were not statistically significant. Overall, there was no significant association between rs1800451 and rs7096206 polymorphism of MBL2 gene and CP.