Journal
ENGENHARIA SANITARIA E AMBIENTAL
Volume 20, Issue 2, Pages 315-321Publisher
ASSOC BRASILEIRA ENGENHARIA SANITARIA AMBIENTAL
DOI: 10.1590/S1413-41522015020000069683
Keywords
biofuel; monoaromatic hydrocarbons; anaerobic biological degradation; real time PCR
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The effects of biodiesel on the biodegradation of benzene and toluene under anaerobic conditions were assessed using two microcosms constructed using synthetic groundwater, methanogenic inoculum and amended with benzene, toluene and biodiesel. In the presence of biodiesel, benzene and toluene degradation was substantially inhibited. Biodiesel degradation was followed by a production of acetate and methane, consumption of sulfate and a 45-fold increase in sulfate-reducing concentration. These results demonstrated that, in the presence of benzene and toluene, biodiesel was biodegraded under sulfate-reduction and methanogenic conditions and also stimulated biomass growth.
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