Journal
BRIEFINGS IN FUNCTIONAL GENOMICS
Volume 17, Issue 4, Pages 233-239Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bfgp/elx035
Keywords
single-cell RNA sequencing; Smart-Seq 2; 10x Chromium; Drop-Seq; experimental design
Funding
- Wellcome Trust [105641/Z/14/Z]
- University of Edinburgh
- Wellcome Trust Strategic Award [105045/Z/14/Z]
- Wellcome Trust [105045/Z/14/Z] Funding Source: Wellcome Trust
- MRC [MC_PC_15075] Funding Source: UKRI
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Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.
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