Journal
ACS OMEGA
Volume 3, Issue 7, Pages 8378-8385Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b00367
Keywords
-
Categories
Funding
- National Institute of Dental and Craniofacial Research, National Institutes of Health (NIDCR/NIH) [R03DE025058-0, R01DE022350, F31 DE025783-01A1]
Ask authors/readers for more resources
Removal of oral biofilms involves the use of broad-spectrum antimicrobials, which eradicate both pathogenic and protective oral commensal species. Ideal therapeutics for dental caries should be able to selectively inhibit pathogenic biofilms caused by Streptococcus mutans. S. mutans extracellular glucosyltransferases (Gtfs), particularly GtfB and GtfC, synthesize predominantly water-insoluble glucans, which contribute to the structural scaffold of biofilms. The lead stilbene identified through our docking study against the catalytic domain of GtfC is a natural product known as piceatannol, which inhibited S. mutans biofilm formation in a dose-dependent manner, with considerable selectivity over growth inhibition of S. mutans and commensal streptococci. Binding kinetic analysis of piceatannol was performed using Octet RED against both GtfB and GtfC, which produced low micromolar K-D values. Piceatannol inhibited S. mutans colonization in an in vivo drosophila model and a rat model of dental caries.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available