Journal
FEMS MICROBIOLOGY LETTERS
Volume 365, Issue 9, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fny069
Keywords
Pseudomonas; aquaculture; whole genome sequencing; Oxford Nanopore MinIon; Ion Torrent; Pseudomonas baetica
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/K019600/1, BB/M009122/1]
- Chief Scientists Office [TCS/16/24]
- Industrial Biotechnology Innovation Centre
- University of Strathclyde
- Iraqi Government
- FWO Vlaanderen [12M4618N, 1S64718N]
- Fundacao para a Ciencia e a Tecnologia [SFRH/BD/104095/2014]
- BBSRC [1654200, 1864373, BB/K019600/1, 1646512] Funding Source: UKRI
- Fundação para a Ciência e a Tecnologia [SFRH/BD/104095/2014] Funding Source: FCT
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Pseudomonas baetica strain a390T is the type strain of this recently described species and here we present its high-contiguity draft genome. To celebrate the 16th International Conference on Pseudomonas, the genome of P. baetica strain a390T was sequenced using a unique combination of Ion Torrent semiconductor and Oxford Nanopore methods as part of a collaborative community-led project. The use of high-quality Ion Torrent sequences with long Nanopore reads gave rapid, high-contiguity and -quality, 16-contig genome sequence. Whole genome phylogenetic analysis places P. baetica within the P. koreensis Glade of the P. fluorescens group. Comparison of the main genomic features of P. baetica with a variety of other Pseudomonas spp. suggests that it is a highly adaptable organism, typical of the genus. This strain was originally isolated from the liver of a diseased wedge sole fish, and genotypic and phenotypic analyses show that it is tolerant to osmotic stress and to oxytetracycline.
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